Surface modification and patterning at nanoscale is science with significant applications in biomedical technology and bioelectronics [1, 2].

The development of methods for patterning and immobilizing biologically active molecules with micrometer and nanometer scale has been proven to be integral to a wide range of applications such as basic research, diagnostics, and drug discovery. Some of the most important advancements have been in the development of biochip arrays that present either DNA [3], protein [4], or carbohydrate [5].

IModification of as surface to anchor protein molecules is an important strategy for obtaining the construction of new biocompatible materials with smart bioactive properties.

Our group is working on pattern the DNA (nanostructred different shapes),cancer antigen-antibodies on mica, and silicon substrates.

REFERENCES

  1. M. Park, C. Harrison, P.M. Chaikin, R.A. Register, D.H. Adamson, Science (Washington DC) 276 (1997) 1401-1404.
  2. R.R. Li, P.D. Dapkus, M.E Thompson, W.G. Jeong, C. Harrison, P.M.Chaikin, R.A. Register, D.H. Adamson, Appl. Phys. Lett. 76 (2000) 1689-1691.
  3. L.M. Demers, D.S. Ginger, S.J. Park, Z. Li, S.W.Chung, C.A. Mirkin, Science (Washington DC) 296 (2002) 1836-1838.
  4. C.D. Hodneland, Y.S. Lee, D.H. Min, M. Mrksich, Proc. Natl. Acad. Sci, 99 (2002) 5048-5052.
  5. B.J. Hohseman, M. Mrksich Chem Biol 9 (2002) 443-454.

TEAM MEMBERS

  1. Dr. S. Kumaran, Senior Lecturer, Department of Biotechnology – PI
    Email: mskumaran@yahoo.com , afm@bio.pmu.edu

Ph.D SCHOLAR (DEPARTMENT OF BIOTECHNOLOGY-SENIOR RESEARCH FELLOW-[CSIR])

  1. Mr. Ramachandra Murthy, K.G., M.Sc. (Materials Science), M.Tech. (Polymer Science and Engineering) - Email: ramacmurthy@yahoo.co.in

JUNIOR RESEARCH FELLOW-DEPARTMENT OF BIOTECHNOLOGY-[DBT]

  1. Ms. Nazeema Banu, M.Tech – Biotechnology - Email: nazeema2020@gmail.com
  2. Ms. Aarthy.R, M. Tech Biotechnology

B.Tech BIOTECHNOLOGY STUDENTS (2007-2008)

  1. Ms. Rajasree (GATE scorer)
  2. Ms. Umamaheswari (GATE scorer)
  3. Ms. V.Maheswari
  4. Ms.P.Gnanambigai

FUNDED PROJECTS

1. SUB-100 NM PATTERNING OF BIOMOLECULES ON SILICON FOR THE NEXT GENERATION CHIP

Funded by Department of Biotechnology, Government of India, New Delhi

Sanctioned Money: 7 million (Indian Rupees)

Sanction Number: (BT/PR10018/NNT/28/95/2007)

Duration: 3 years (2008-2011)

Dr. S. Kumaran, Senior Lecturer, Department of Biotechnology

Co-Investigators:
  • Dr. K. Rajendran, Assistant Professor, Department of Biotechnology.Email:nkraj64@yahoo.co.uk
  • Dr. T.Eevera, Assistant Professor, Department of Biotechnology,
  • Dr. D. Kumar, H.O.D, Electronics & Communication Engineering, and Dean (Research), Email:deanresearch@pmu.edu
  • Dr. A. Kathalingam, Assistant Professor, Department of Physics, Head-Nanotechnology group,

Project description: Fabrication of Self Assembled Monolayers (SAMs), N-type Si (111) surfaces will be oxidized with the SC1 process1, and will be modified with octadecyltrichlorosilane (OTS). Oxide dots will be fabricated by applying a bias voltage between the AFM probe and the surface. Amino groups and aldehyde groups will be attached to the surface for anchoring biomolecules. Topographies after each modification steps will be monitored by AFM2, 3.

References:
  1. H. Sugimura and N. Nakagiri, J. Am. Chem. Soc. 119, 9226 (1997).
  2. T. Yoshinobu, J. Suzuki, H. Kurooka, W.C. Moon, H. Iwasaki, Electrochim. Acta. 48, 3131 (2003).
  3. J. Suzuki, T.Yoshinobu, W. Moon, K. Shanmugam and H.Iwasaki, Electrochemistry, 74, 131 (2006)

2. NANOLEVEL IMAGING OF ANTIGEN-ANTIBODY INTERACTIONS USING AFM

Funded by Tamil Nadu Science & Technology, Chennai (Madras)

Sanctioned Money: Rs. 5000/- (Eventhogh this is very small amount, we are one of the recipients from state level competition)

Letter Number: TNSCST/STU.PRJ/RJ/2007-08/

Final year B.Tech Biotechnology students project (Jan 2008-May 2008)

Project description: : We are planning to immobilize antibodies-antigen on substrates like mica, and membrane. The images shown below are taken using AFM. Fabrication of nanostructure DNA.

Equipment: -

GROUP MEETING

Every Thursday between 9.15 a.m to 9.45 a.m; if Thursday is holiday, meeting will be conducted on the next working day.

PUBLICATIONS (SELECTED PUBLICATIONS OUT OF 14)

  • Positive Patterning of Ferritin, and Fibronectin Molecules on Silicon by Atomic Force Microscopy Anodic Oxidation, Kumaran Shanmugam, Tatsuo Yoshinobu, Wonchul Moon, Hiroshi Iwasaki (manuscript in preparation). [Conference abstract]
  • Polarographic and voltammetric determination of submicromolar concentrations of 1,8-DNN, Kumaran Shanmugam, Jirí Barek and Jirí Zima (manuscript in preparation). [Final report to Czech Grant Agency]
  • Micropatterning of Si Surface with Protein Molecules by the AFM Anodic Oxidation Method, Jun Suzuki, Tatsuo Yoshinobu, Wonchul Moon, Kumaran Shanmugam and Hiroshi Iwasaki, Electrochemistry, 74, 131-134, 2006. [PDF]
  • Voltammetric determination of Phenylglyoxylic Acid in Urine using Graphite Composite Electrode, Thomas Navratil, Zdenka Senholdova, Kumaran Shanmugam, and Jiri Barek, Electroanalysis, 18, 2, 201-206, 2006. [PDF]
  • Polarographic and voltammetric determination of trace amounts of 1, 3-dinitronaphthalene, Kumaran Shanmugam, Jiri Barek and Jiri Zima, Chem. Anal. (Warsaw), 49, 765-776, 2004. [PDF]
  • Polarographic and voltammetric determination of submicromolar concentrations of genotoxic 1,5-dinitronaphthalene, Kumaran Shanmugam, Jiri Barek and Jiri Zima, Czechoslovak Chemical Communication, 69, 2021-2035, 2004. [PDF]
  • 7. 2,4-Toluene Diamines – Their Carcinogenicity, Biodegradation, and Analytical Techniques and an Approach towards Development of Biosensors -A General Review, Kumaran Shanmugam, Sreenath Subrahmanyam, Subramanian V. Tarakad, Narendran Kodandapani, and D’Souza F. Stanly, Analytical Sciences,17, 1369-1374, 2001. [PDF]

CONFERENCE PRESENTATIONS (SELECTED PUBLICATIONS OUT OF 17)

  • Positive patterning of ferritin, and fibronectin molecules on silicon by atomic force microscopy anodic oxidation, Kumaran Shanmugam, Tatsuo Yoshinobu, Wonchul Moon, and Hiroshi Iwasaki, National conference on Environmental Challenges and ManagementECM-2007) on 9th of March 2007 at Bharathiar University, Coimbatore, India. (Invited) Polarographic and voltammetric determination of genotoxic dinitronapthalenes, Kumaran Shanmugam, Jirí Barek, and Jirí Zima. (Oral)
  • Positive patterning of ferritin and fibronectin molecules on silicon by AFM anodic oxidation technique, Kumaran Shanmugam, Tatsuo Yoshinobu, Wonchul Moon, Hiroshi Iwasaki, International Conference on Nanomaterials & its Applications (ICNA-2007) on 4th-6th February, 2007 at National Institute of Technology (NIIT), Tiruchirapalli, India.Invited)
  • Positive patterning of ferritin and fibronectin molecules on silicon, Kumaran Shanmugam, Tatsuo Yoshinobu, Hiroshi Iwasaki, Montpellier 2006, June 3-6, France. (Poster)
  • Positive Patterning of ferritin and fibronectin molecules on Silicon, Kumaran Shanmugam, Tatsuo Yoshinobu, Hiroshi Iwasaki, COE symposium, ISIR-Bio Nano group presentation on 3rd -4th March 2006, Westrin Hotel, Awaji Island, Japan. (Oral)
  • Positive patterning of ferritin and fibronectin molecules on Silicon, Kumaran Shanmugam, Tatsuo Yoshinobu, Hiroshi Iwasaki, Handai Nanoscience and Nanotechnology International Symposium. January 30-February 1, 2006, Osaka University, Japan. (Poster)
  • Positive Patterning of Ferritin Molecules on Silicon, Kumaran Shanmugam, Tatsuo Yoshinobu, Hiroshi Iwasaki, Cancun 2005, Scanning Probe Microscopy, Sensors and Nanostructures, Omni Cancun Hotel and Vilas, Mexico, June 5th to 8th 2005. (Poster)
  • Voltammetric determination of one of styrene metabolites-Phenylglyoxylic acid- in Urine Using Graphite Composite Electrode, Tomáš Navrátil, Zdenka Šenholdová, Kumaran Shanmugam, Jirí Barek, and Helena Surovocoá, XXV Moderní Elektrochmrické metody, 24-26, May 2005, Jetrichovice, Czech Republic. (Oral)
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